A hot air oven is used to sterilize equipment and materials used in the medical field. Microbiology equipment is a large category covering all kinds of items used in microbiology laboratories. Pdf an assignment of microbiology laboratory use of. Medical laboratory is one part of the laboratory that is equipped with various biomedical instruments, equipments, materials and reagents chemicals. Laboratory safety equipment biological safety cabinet a biological safety cabinet bsc is used as a primary barrier against exposure to infectious biological agents. However these principles could be applied in the maintenance of other equipment in the laboratory as well. Maintenance of laboratory equipment this guideline deals with some equipment commonly used in the microbiology laboratories.
An emphasis is placed on the basic principles of diagnostic microbiology and the lab procedures used for isolation and identification of infectious agents. It is used for sterilization of glasswares, such as test tubes, pipettes and petri dishes. Laboratory experiments in microbiology ninth edition. Sterilisation of media, solutions and equipment before use and contaminated. Such dry sterilization is done only for glasswares. Microbiology module sterilisation and disinfection microbiology 44 notes 1. Phenol solutions can be used for decontaminating equipment. These include procedure manuals, maintenance schedules, calibrations, a quality assurance program, training and quality control. Some of the glassware instruments used in microbiology laboratory are. Laboratory experiments in microbiology 11th edition pdf. There microscopes used in an introductory microbiology laboratory is a compound light or brightfield microscope.
Laboratory facilities equipment incubation conditions disposal procedures expertise of technicians and teachers student age and discipline sources of competent advice useful check list essential reference risk assessment. This method applies a chemical in a gaseous state to destroy micro bacteria. An introduction to microbiology, aseptic technique and safety preparation safety guidelines 1 risk assessment 2 good microbiological laboratory practice gmlp 3 spillage management 3 aerosols 3 resources equipment 4 apparatus 5 materials 5 media, sterilisation and disinfection preparation of culture media 6 pouring a plate 6 storage of media 6. As always you should test labware to ensure product compatibility. Quality production laboratory materials facilities and equipment packaging and labeling. A laboratory manual pdf using our direct download link. Bunsen burner bunsen burner is a common tool used in science lab fig. Basic practical microbiology why microbiology matters.
The goal of the laboratory is to expose students to the wide variety of lives in the microbial world. The management should recruit qualified and experienced people to work in the microbial laboratory. Alcohols can be used on skin, work surfaces of laboratory benches and biosafety cabinets, and to soak small. Also learn about the process of handling and caring of laboratory glasswares. Safety in a microbiology laboratory is important in the prevention of infection as microbiology laboratory cultures, manipulates, and uses virulent andor potentially pathogenic microorganisms. Dry heat sterilization is used on equipment that cannot be wet, and on material that will not melt, catch fire, or change form when exposed to high temperatures. Microbiology is the study of microscopic life forms and is used by scientists studying viruses, plants, fungi, protozoans, cells, and parasites. Table 2 provides a more detailed description on the application of these standards and examples of laboratory coats that meet these standards. Olaf college, a liberal arts college in northfield, minnesota, where he teaches courses in microbiology and immunology. Each peice of equipment has a picture, a description, and a list of uses for the equipment. Proper recordkeeping is another important facet of good laboratory protocol.
Key lessons for work flow, analyzers, and service improvements atlanta, ga purposeobjectives purpose. Food safety is a key topic of concern among consumers and the future of food safety starts with testing in the laboratory or on the field. A compound microscope consists of at least two magnifying lenses. It includes just about every type of lab equipment that is used in a chemistry course. Absolutely no food, drinks, chewing gum, or smoking is allowed in the laboratory. Maintenance of laboratory equipment it is intended to. This system is used for controlled encapsulation of bioactives and materials for.
This equipment is used in microbiology laboratory for storing preserving cultures, media, and many sensitive materials fig. Chemicals that require labels, should have labels that are legible. But one needs to maintain minimum sterilization time or minimum contact time for the heat to be in touch with microbes or bacteria and thereby kill them the heat method of sterilization is again of two types based on the type of heat used. In the laboratory, eight main types of instruments are used. These topics include nucleic acid amplification tests such as isothermal and pointofcare molecular diagnostics, multiplexed panels for syndromic diagnosis, digital pcr, nextgeneration sequencing, and automation of molecular tests. A list of chemistry laboratory apparatus and their uses. Despite heat sterilization is unstable for thermolabile equipment like delicate, plastic and rubber items, it has become popular due to its capital cost. All light microscopes have the same basic features shown in figure 1. Procedures in the clinical microbiology laboratory clsi m52p. A detailed discussion of the different types of physical and chemical methods of sterilization is provided in chapter 19 physical and chemical methods of control. A hot air oven is a type of dry heat sterilization.
Laboratory manual and workbook in microbiology applications to patient care 7th edition. This chapter offers a concise overview of equipment and instrumentation thats frequently used in microbiology labs. Laboratory experiments in microbiology 11th edition pdf kindle free download. Food testing equipment food and beverage testing lab equipment is used to evaluate the quality and integrity of solid and liquid food samples. A modern microbiology laboratory should be furnished with the following equipment. List of equipment apparatus used in microbiology laboratory. Standard safety practices in the microbiology laboratory appendix 1. Standard safety practices in the microbiology laboratory. To accomplish this, the corporate management team makes the necessary financial resources for the equipment and staffing needed to ensure the following quality. Glassware and instruments used in microbiology laboratory. They are used to heat on hold reagents for observing. A read is counted each time someone views a publication summary such as the title, abstract, and list of authors, clicks on a figure, or views or downloads the fulltext.
Standard operating procedure sop for laboratory disinfection issued. Representative sample consists of a number of units that. Which of the following types of personal protective equipment ppe is frequently used. Emerging technologies for the clinical microbiology laboratory. This is a very detailed laboratory equipment reference chart. Laboratory is a place that is equipped with different instruments, equipments and chemicals reagents etc. In addition to microorganisms, there are some chemicals used in this laboratory that are potentially harmful. Equipment used in the manufacture, processing, packing, or holding of a drug. Equipment, materials, and supplies always must be in their proper place. Module context this module introduces microbiological laboratory techniques to be used for analysis of coliforms bacteria can be used as indicators of pollution. If youre looking for a free download links of laboratory experiments in microbiology 11th edition pdf, epub, docx and torrent then.
By advancement in technology to make the laboratory testings more accurate, fast, reliable and cost effective, we require these instruments with its proper care. Verification and validation in clinical microbiology. To provide an overview of using six sigma and lean principles in chartering, designing and implementing a full scale lean project in a large laboratory setting. The most commonly used instrument in the microbiology laboratory for the sterilization of media and glassware is the autoclave. We do not imply these products are fit for any particular use. The maintenance programme to be developed in the laboratory should include the following components. Using six sigma and lean principles to improve laboratory. List of equipment apparatus used in microbiology laboratory 1. The components of good housekeeping in a laboratory are discussed. General laboratory stands, racks, filter paper, reagents. Table 1 lists the three types of laboratory hazards and the applicable laboratory coat material standards. Instruments techniques and principals in microbiology 1.
Basic laboratory procedures in clinical bacteriology. A lab funnel is just like any other funnel except that it was designed to be used in a laboratory setting. The equipment is electrically powered and uses ammonia as the refrigerant. Instruments used in medical laboratories wikipedia. A bsc has high efficiency particulate air hepa filters. Further, the sterilization time increases with a decrease in temperature and viceversa. They can be made of plastic or glass and can have either a short stem or a long stem, depending on what they are needed for. An assignment of microbiology laboratory use of microbiology lab, chemical and equipment. Who good practices for pharmaceutical microbiology laboratories. Read online laboratory experiments in microbiology ninth edition laboratory experiments in microbiology ninth edition laboratory experiments in microbiology ninth ted r. Pdf on oct 10, 2008, abu nayeem and others published an.
The performance characteristics being verified can include. Instruments used specially in microbiology include. Wash your hands with disinfectant soap when you arrive at the lab and again before you leave. Alright, now in this part of the article, you will be able to access the free pdf download of microbiology. Instrumen t s used specially in microbio log y include. Laboratory equipment used in the microbiology laboratory. Validation and verification of id and ast systems not yet published definitions as used by clia clsi verification a onetime process completed before a test or instrument is used for patient testing. Although the study of microbiology includes bacteria, viruses, algae and protozoa, this lab will concentrate primarily on the bacteria. Sks science offers several models of affordable yet highquality lab equipment items that could be useful in a microbiology lab, including digital pipette controllers and magnetic stirrers. Microbiology lab safety university of minnesota duluth. The staff, environment, test method validation, equipment used, reagents and media used and referencing to international standards are the key aspects of glp regulations. Microbiological laboratory techniques clinical gate. Free torrent download laboratory experiments in microbiology 11th edition pdf ebook. Steam under pressure is used to generate high temperature needed for.